论文部分内容阅读
本试验以从张家口市黑龙山地区采集的40个榆树无性系为试验材料,对叶形性状和SSR分子标记进行关联分析。结果表明,在40个榆树无性系间,15个叶形性状均有着极显著变异;大多数叶形指标的重复力均在0.8以上。利用15个叶形性状对40个榆树无性系进行聚类分析,可分为4类,各无性系间遗传距离在0.578~9.309之间。以从17对引物中选出的13对多态性高的SSR引物对40个榆树无性系进行聚类分析,可完全分为4类,各无性系间的遗传距离在0.030~0.664之间。15个叶形性状与SSR分子标记达到极显著相关,相关系数为0.473 8(p<0.001,n=780,rα=0.118)。这说明,叶形性状与所选SSR位点关联水平较高。
In this study, 40 elm clones collected from Heilongshan of Zhangjiakou City were used as experimental materials to analyze the leaf traits and SSR molecular markers. The results showed that among the 40 elm clones, all 15 leaf traits showed extremely significant variation. The repeatability of most leaf index was above 0.8. Cluster analysis of 40 elm clones using 15 leaf traits can be divided into four categories, and the genetic distance between the clones was between 0.578 and 9.309. Thirteen pairs of SSR primers with high polymorphism were selected from 17 pairs of primers to classify 40 elm clones. They could be completely divided into 4 groups. The genetic distance between clones was between 0.030 and 0.664. The 15 leaf traits were significantly correlated with SSR markers with a correlation coefficient of 0.473 8 (p <0.001, n = 780, rα = 0.118). This suggests that leaf traits are associated with higher SSR loci.