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以从云南省主要烟区烟草上分离到的烟草花叶病毒 (Tobacco mosaic virus,TMV)和黄瓜花叶病毒 (Cucumber mosaic virus,CMV)的 RNA为模板 ,采用 RT- PCR技术 ,对 TMV云南分离物 (TMV-YN)的外壳蛋白 (Coatprotein,CP)基因及 3’端非编码区和 CMV云南分离物 (CMV- YNa)的 CP基因进行了 c DNA克隆和序列测定 .结果表明 ,TMV- YN的 c DNA全长 6 84个碱基 (EMBL登录号为AJ2 390 99) ,通过 Gen Bank进行同源性比较发现 :其 5’端的 4 80个碱基为 CP基因 ,编码 159个氨基酸 ;3’端的 2 0 4个氨基酸为非编码区 ;此 CP基因与 TMV- U1株系和 TMV韩国普通株系核苷酸同源性均为 10 0 % . CMV- YNa的 CP基因全长 6 57个碱基 (EMBL登录号为 AJ2 390 98) ,编码 2 18个氨基酸 ;此CP基因与 CMV亚组 I的 CMV- RB株系、CMV- 117F株系和 CMV- Y株系的核苷酸同源性分别为96 %、93%和 92 % .
The RNA of Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) isolated from tobacco in Yunnan province was used as a template to isolate TMV Yunnan (CP) gene of CPV (TMV-YN) and CP gene of CMV Yunnan isolate (CMV-YNa) were cloned and sequenced.The results showed that TMV-YN The full length cDNA of c DNA was 6 84 bp (EMBL accession number AJ2 390 99). Genomic comparison of GenBank revealed that the 4 80 bases at the 5 ’end were CP gene and encoded 159 amino acids. The 3’ The nucleotide of the CP gene was 100% homologous to TMV-U1 strain and TMV Korea common strain.The CP gene of CMV-YNa had a total length of 6 57 bases (EMBL accession number AJ2 390 98) encoding 2 18 amino acids; this CP gene shares nucleotide homology with the CMV-RB, CMV-117F and CMV-Y strains of the CMV subgroup I Respectively 96%, 93% and 92%.