论文部分内容阅读
目的探讨广州地区儿童感染幽门螺杆菌(H.pylori)的iceA基因亚型与胃炎的关系。方法105例胃、十二指肠疾病患儿的胃窦处取3块胃黏膜,分别进行快速尿素酶反应、病理检查和聚合酶链反应(PCR)。抽提胃黏膜基因组DNA,用3对引物检测H.pyloriureA和iceA基因,分析H.pylori iceA基因亚型与胃炎的关系。结果105例样本中,快速尿素酶反应、病理检测和聚合酶链反应(PCR)三者均阳性的标本52例,H.pylori iceA1亚型菌株单独检出率78.84%(41/52),H.pylori iceA2亚型菌株单独检出率1.92%(1/52),H.pylori iceA1和iceA2亚型均阳性的检出率3.84%(2/52),iceA1和iceA2亚型均阴性的比率15.38%(8/52),H.pylori iceA1亚型阳性率与其它基因亚型相比较,差异有显著性;感染H.pylori iceA1亚型菌株的患儿中轻度慢性胃炎3例,中度慢性胃炎15例,重度慢性胃炎25例;未感染H.pylori iceA1亚型菌株的患儿中轻度慢性胃炎2例,中度慢性胃炎4例,重度慢性胃炎3例,感染H.pylori iceA1亚型菌株患儿与未感染H.pylori iceA1亚型菌株患儿的胃黏膜病变程度差异有显著性(P<0.05)。结论H.pylori iceA1亚型是广州地区儿童感染H.pylori的优势基因亚型,H.pylori iceA1菌株容易引起较严重慢性胃炎。
Objective To investigate the relationship between iceA gene subtypes and gastritis in children infected with H.pylori in Guangzhou. Methods Three gastric mucosa were taken from the gastric antrum of 105 children with gastric and duodenal diseases. Rapid urease reaction, pathological examination and polymerase chain reaction (PCR) were performed respectively. Gastric mucosa genomic DNA was extracted and three pairs of primers were used to detect H.pyloriureA and iceA genes. The relationship between H.pylori iceA subtypes and gastritis was analyzed. Results Among the 105 samples, 52 were positive for rapid urease, pathology and polymerase chain reaction (PCR). The positive rate of H.pylori iceA1 isolates was 78.84% (41/52), H The positive detection rate of H.pylori iceA1 and iceA2 was 3.84% (2/52), the negative rate of both iceA1 and iceA2 was 15.38 % (8/52). The positive rate of H.pylori iceA1 subtype was significantly higher than that of other subtypes. In the children infected with H.pylori iceA1 subtype, there were 3 mild chronic gastritis, moderate chronic Gastritis in 15 cases and severe chronic gastritis in 25 cases. There were 2 cases of mild chronic gastritis, 4 cases of moderate chronic gastritis and 3 cases of severe chronic gastritis without H.pylori iceA1 subtype. There was a significant difference in gastric mucosal lesions between children with and without H.pylori iceA1 subtype (P <0.05). Conclusions The H.pylori iceA1 subtype is the predominant subtype of H.pylori infection in children in Guangzhou, and the H.pylori iceA1 strain can easily cause more severe chronic gastritis.