【摘 要】
：
目的 观察淫羊藿甙对体外培养大鼠成骨细胞丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路的影响以及MAPK信号通路在淫羊藿甙促成骨细胞核心结合因子α
论文部分内容阅读
目的 观察淫羊藿甙对体外培养大鼠成骨细胞丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路的影响以及MAPK信号通路在淫羊藿甙促成骨细胞核心结合因子α1(core binding factor-1,Cbfa1)蛋白表达中的作用,以探讨淫羊藿甙对成骨细胞作用的信号传导机制.方法 用酶消化法分离24 h内新生SD大鼠颅盖骨成骨细胞,进行原代培养.在培养液中加入淫羊藿甙(10 ng/mL),作用于成骨细胞5 min、10 min、30 min、60 min,抽提总蛋白,用Western blot法检测细胞中ERK、p-ERK、P38和p-P38蛋白的表达.用雌二醇(10-8 mol/L)作用于成骨细胞5 min、10 min、30 min,同上法检测细胞中ERK、p-ERK、P38和p-P38蛋白的表达.用淫羊藿甙(10 ng/mL)、雌二醇(10-8 mol/L)和u0126或SB203580单独或共同干预成骨细胞24 h,抽提核蛋白,用Western blot法检测Cbfa1蛋白的表达.结果 ①淫羊藿甙作用于成骨细胞,30 min时可促进ERK蛋白的磷酸化,并可持续至60 min,和空白组比较,差异有显著性(P
其他文献
In this article, the authors obtain an inequality of homogeneous expansion for f, where f is a quasi-convex mapping (including quasi-convex mapping of type A an
In the study of process calculi, encoding between different calculi is an effective way to compare the expressive power of calculi and can shed light on the ess
It is showed that,as the Mach number goes to zero,the weak solution of the compressible Navier-Stokes equations in the whole space with general initial data con
A simple robust"strut algorithm"is presented which, when given a graph embedded in 3D space, thickens its edges into solid struts. Various applications, crystallographic and sculptural, are shown in w
The title compounds,C7H4I2O2 1 and C13H7I2N2O3 2,have been synthesized and characterized by single-crystal X-ray diffraction.Compound 1 crystallizes in monoclin
Monolithic catalysts of Pt/La-Al2O3 and Pt/Ce0.67Zr0.33O2 were prepared to investigate methane selective catalytic reduction (SCR) of NO.The results indicate th
In this article, the authors define the derived function of an algeboidal function in the unit disc, prove it is an algabriodal function, and study the order of
Chiral bis-corrole 1 and 2 were prepared by reaction of (s)-2,2-bis(bromomethyl)-1,1-binaphthyl and 10-(4-hydroxylphenyl)-5,15-bis(pentafluorophenyl)corrole or
目的 了解低出生体重儿(LBWI)生后1岁内追赶性生长、体格发育速度和营养性疾病的发病情况. 方法 将1岁内于2、4、6、9、12月龄进行系统体检的100例LBWI作为低体重组,与93例正
溶组织内阿米巴是HIV/AIDS患者常见的机会感染病原体之一,是HIV感染者腹泻的主要原因,可引起肝脓肿、脑脓肿及浆膜腔脓肿等危及患者生命的并发症.