论文部分内容阅读
本文以大豆粉作为主要的研究对象，重点阐述了大豆异黄酮在经过一系列提取后的纯化效果，且对10% 纯度的大豆异黄酮原粉对小鼠衰老指标的影响进行了深入分析。从最终的试验结果来看，60% 浓度的乙醇以及其他物料之间的比例为1:5，在60℃的水浴条件下经过4 个小时的提取，提取出1.61% 纯度的大豆异黄酮粗提物，后经水解处理与树脂层析分离后将会获得纯度为10% 的大豆异黄酮原粉。在将其应用至小鼠身上后，能够从根本上将小鼠的过氧化脂质含量降低，且能够进一步提升谷胱甘肽过氧化物的酶活性，皮肤羟脯氨酸的含量相较以往也有了明显提升，继而判定大豆异黄酮能够起到一定的rn抗衰老作用。“,”This article takes soybean powder as the main research object, focusing on the purification effect of soybean isoflavones after a series of extractions, and the effect of 10% pure soybean isoflavones on the aging indicators of mice analysis. From the final test results, the ratio between 60% ethanol and other materials is 1:5. After 4 hours of extraction at 60℃ in a water bath, 1.61%rnpure soybean isoflavones are extracted. After being hydrolyzed and separated by resin chromatography, soy isoflavone raw powderrnwith a purity of 10% will be obtained. After applying it to mice, it can fundamentally reduce the lipid peroxide content of mice, and canrnfurther increase the enzyme activity of glutathione peroxidase. The content of skin hydroxyproline is higher than before There was also arnsignificant improvement, and it was determined that soy isoflavones can play a certain anti-aging effect.