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目的观察大鼠海马神经元在激活物作用下γ-氨基丁酸(GABAA)受体的表达,进一步探讨癫发病机制。方法将大鼠海马神经元被戊四氮(PTZ)作用后的激活物(实验组)及无血清培养基(对照组)注入大鼠侧脑室后观察其行为、脑电图(EEG)及脑组织GABAA受体表达的变化。结果实验组大鼠注射后15~30min出现Ⅱ~Ⅲ级惊厥反应,EEG呈短程中高幅尖波、尖慢复合波;对照组的行为及EEG未见异常;各时点实验组大鼠脑组织GABAA免疫反应阳性细胞表达明显低于对照组(均P<0.05),对照组GABAA免疫反应阳性细胞广泛分布于大脑皮质、海马回、齿状回。结论海马神经元激活物具有明显致疒间作用,其致疒间效应与GABAA受体含量下降有关。
Objective To investigate the expression of γ-aminobutyric acid (GABAA) receptors in rat hippocampal neurons under the action of activators, and to further explore the pathogenesis of epilepsy. Methods The hippocampal neurons were injected into the lateral ventricle of rats after activation by pentylenetetrazole (experimental group) and serum-free medium (control group), and their behaviors, electroencephalogram (EEG) Changes in tissue GABAA receptor expression. Results The rats in experimental group showed grade Ⅱ ~ Ⅲ seizure reaction 15 ~ 30 minutes after injection, and EEG showed short-range mid-high amplitude sharp wave and sharp slow wave complex. The behavior and EEG of control group showed no abnormality. At each time point, The expression of GABAA immunoreactive cells was significantly lower than that of the control group (all P <0.05). The GABAA immunoreactive cells in the control group were widely distributed in cerebral cortex, hippocampus and dentate gyrus. Conclusion The hippocampal neuron activator has a significant synergistic effect, and its ployer effect is related to the decrease of GABAA receptor.