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目的 研究急性乙醇摄入与内源性一氧化氮 (NO)水平变化的关系。方法 3 5只大鼠随机分成A、B、C三组 ,其中A组 (n =11)为对照组 ,以生理盐水 0 5g/kg尾静脉注入 ;B (n =12 )、C (n =12 )组分别以无水乙醇按 0 3和 0 7g/kg从大鼠尾静脉注入 ,取血标本用于测定血清中NO水平和丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶(AST)活性。结果 (1)A、B组大鼠无明显生物行为上的改变 ;C组大鼠其中 3只即刻呼吸心跳停止 ,经心肺复苏无效而死亡。 (2 )A、B、C三组血清NO水平分别为 (3 9 2 7± 3 2 5 )、 (4 2 3 0± 4 60 )和 (4 7 86± 4 66) μmol/L ,C组与A、B组相比 ,血清NO水平差异分别具有非常显著意义 (P≤ 0 0 1)。A、B、C三组血清ALT水平分别为 (74 2 7± 3 3 17)、(73 5 8± 11 70 )和 (81 67± 2 3 2 9)U/L ,各组间差异无显著意义 ;AST水平分别为 (182 82± 10 1 68)、 (186 3 3± 3 8 0 7)和(2 5 1 5 6± 99 84)U/L ,C组与B组间差异有显著性 (P≤ 0 0 5 ) ;AST/ALT比值分别为 2 44± 0 46,2 5 7± 0 60和 3 0 3± 0 46,C组与A、B组相比 ,差异分别具有非常显著和显著意义 (P≤ 0 0 1,P≤ 0 0 5 )。结论 大鼠急性乙醇摄入可引起肝脏损害及NO水平改变 ,并与AST/ALT比值呈正相关。
Objective To investigate the relationship between acute alcohol intake and changes of endogenous nitric oxide (NO) levels. Methods A total of 5 rats were randomly divided into three groups (A, B and C). The rats in group A (n = 11) were injected with 0.5 g / 12) were injected into the caudal vein of rats with 0 3 and 0 7 g / kg of absolute ethanol, respectively. Blood samples were taken for the determination of serum NO levels and alanine aminotransferase (ALT), aspartate aminotransferase (AST) active. Results (1) There was no significant change in the biological behavior of rats in groups A and B; three of the rats in group C stopped breathing immediately and died of cardiopulmonary resuscitation failure. (2) The levels of serum NO in group A, B and C were (3927 ± 325.5), (4220 ± 460) and (4768 ± 446) μmol / L, Compared with A and B groups, the differences of serum NO levels were very significant (P≤0 0 1). The levels of serum ALT in groups A, B and C were (74 2 7 ± 3 3 17), (73 5 8 ± 11 70) and (81 67 ± 2 3 2 9) U / L, respectively, with no significant difference between groups (182 82 ± 10 1 68), (186 3 3 ± 3 8 0 7) and (2 5 1 56 ± 99 84) U / L, respectively. There was a significant difference between group C and group B (P≤0 05). The AST / ALT ratio was 444 ± 46,2 57 ± 60 and 300 ± 46 respectively. There was a significant difference between group C and group A and group B Significant significance (P≤0 0 1, P≤0 0 5). Conclusion Acute ethanol intake in rats can cause liver damage and NO level changes, and is positively correlated with AST / ALT ratio.