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目的:探讨健康人和胃癌患者细胞因子诱导的杀伤细胞(CIK)对原代癌细胞的体外抗肿瘤作用的差异,进一步了解CIK细胞的抗肿瘤作用。方法:分离健康人和胃癌患者自身的外周血单个核细胞,分别加入鼠抗人CD3 McAb、基因重组人IL-1α、IFN-γ、IL-2,经体外培养诱导为CIK细胞;手术留取的新鲜胃癌组织用机械研磨法分离成单细胞悬液;用四甲基偶氮唑盐(MTT)法检测2种CIK细胞对胃癌细胞的杀伤活性。结果:健康人的CIK细胞较胃癌患者自身的CIK细胞的体外增殖力强,第1天表达CD3+/CD56+的细胞健康人占(1.064±0.22)%,癌患者占(1.031±0.13)%;而经细胞因子刺激后的第7天健康人占(28.66±2.00)%,癌患者占(17.63±2.22)%;14 d后分别占(57.14±1.96)%和(48.53±2.19)%(P<0.05);其杀伤肿瘤的能力也远远高于患者自身的杀伤能力。结论:健康人和胃癌患者的CIK细胞对原代癌细胞都有细胞毒的作用,健康人的CIK细胞无论增殖能力和细胞毒的活性都远高于肿瘤患者自身的CIK细胞。
Objective: To investigate the antitumor effects of cytokine-induced killer cells (CIK) on primary cancer cells in healthy and gastric cancer patients in vitro and to further understand the anti-tumor effect of CIK cells. Methods: Peripheral blood mononuclear cells were isolated from healthy volunteers and patients with gastric cancer. Mouse anti-human CD3 McAb, recombinant human IL-1α, IFN-γ and IL-2 were added respectively and induced by CIK cells in vitro. Fresh gastric cancer tissue was separated into single cell suspension by mechanical grinding method. The killing activity of the two kinds of CIK cells on gastric cancer cells was detected by MTT assay. Results: CIK cells in healthy individuals had stronger proliferative ability than CIK cells in gastric cancer patients. The percentage of CDK positive cells was (1.064 ± 0.22)% on day 1 and (1.031 ± 0.13)% on cancer cells. On the 7th day after stimulation with cytokines, the percentage of healthy persons was (28.66 ± 2.00)% and that of cancer patients was (17.63 ± 2.22)%, respectively; it was 57.14 ± 1.96% and 48.53 ± 2.19% respectively after 14 days (P < 0.05); its ability to kill the tumor is much higher than the patient’s own ability to kill. CONCLUSION: CIK cells from healthy and gastric cancer patients have cytotoxic effects on primary cancer cells. CIK cells from healthy people have much higher proliferative ability and cytotoxic activity than CIK cells from cancer patients.