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荧光漂白恢复(Fluorescence PhotobleachingBecovery,FPR)技术是采用强激光瞬时漂白荧光标记的细胞膜微区,然后用弱激光记录被漂白区域中荧光恢复的过程,获得荧光漂白恢复动力学曲线,由此计算扩散系数或流动速度.我们曾于1981年研制了FPR装置,并用于测定了林蛙卵表面受体的运动,以了解膜运动与细胞分裂的关系.由于FPR测量范围小于10μm~2的微区而难于了解卵表面大区域的流动变化.鉴于上述原因,我们在原有
Fluorescence Photobleaching Recovery (FPR) is a technique that uses the intense laser instantaneously bleached fluorescently labeled cell membrane micro-regions and then uses a weak laser to record the fluorescence recovery in the bleached region to obtain the fluorescence bleaching recovery kinetic curve, thereby calculating the diffusion coefficient Or flow rate.We developed the FPR device in 1981 and used it to measure the movement of Rana egg surface receptors to understand the relationship between membrane movement and cell division.Due to the FPR measurement range of less than 10μm ~ Understand the changes in the flow of large areas of the egg surface. In view of the above reasons, we are in the original