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目的观察适量运动和甲状腺激素对大鼠血液H2O2含量和抗氧化能力的影响。方法将64只Wistar大鼠随机分为8组:对照组(C)、轻度甲亢组(T1)、重度甲亢组(T2)、甲减组(J)、运动组(E)、轻度甲亢运动组(ET1)、重度甲亢运动组(ET2)、甲减运动组(EJ)。C组生理盐水灌胃,T1和T2组分别给予左旋甲状腺素片100和200μg/d灌胃,J组给予甲巯咪唑10 mg/d灌胃,运动为负重(6%体重)游泳训练30 min/d。喂养14 d后,检测全血中的H2O2含量、谷胱甘肽过氧化酶(GPx)活力、红细胞超氧化物歧化酶(SOD)活力以及血浆丙二醛(MDA)含量。结果与对照组比较,甲亢组H2O2含量、GPx活力、SOD活力和MDA含量均升高,差异有统计学意义(P<0.05);甲减组H2O2含量、GPx活力、SOD活力和MDA含量均降低,差异有统计学意义(P<0.05);与相应非运动组比较,运动组H2O2含量和GPx活力增高,SOD活力和MDA含量降低,差异有统计学意义(P<0.05)。结论运动和甲亢均可诱发全血H2O2含量和抗氧化酶GPx活力增高,而且甲亢会诱发氧化损伤,但适量运动可减轻氧化损伤。大鼠血液对运动氧应激反应代偿作用强于甲亢时。
Objective To observe the effects of moderate exercise and thyroid hormones on blood H2O2 content and antioxidant capacity in rats. Methods Sixty - four Wistar rats were randomly divided into 8 groups: control group (C), mild hyperthyroidism group (T1), severe hyperthyroidism group (T2), hypothyroidism group (J), exercise group (E), mild hyperthyroidism Exercise group (ET1), severe hyperthyroidism exercise group (ET2), hypothyroidism group (EJ). Rats in group C were given intragastric administration of normal saline. T and T2 groups were given 100 and 200 μg / d of levothyroxine tablets respectively. Group J was given 10 mg / d of methimazole orally and exercise was loaded with 6% body weight for 30 min / d. The levels of H2O2, glutathione peroxidase (GPx), erythrocyte superoxide dismutase (SOD) and plasma malondialdehyde (MDA) contents in whole blood were measured 14 days after feeding. Results Compared with the control group, the levels of H2O2, GPx, SOD and MDA in hyperthyroidism group were significantly increased (P <0.05). The levels of H2O2, GPx, SOD and MDA in hypothyroidism were decreased (P <0.05). Compared with the corresponding non-exercise group, exercise group H2O2 content and GPx activity increased, SOD activity and MDA content decreased, the difference was statistically significant (P <0.05). Conclusion Both exercise and hyperthyroidism can induce H2O2 content and GPx activity in whole blood, and hyperthyroidism can induce oxidative damage, but moderate exercise can reduce oxidative damage. Rat blood to exercise oxygen stress response compensatory effect stronger than hyperthyroidism.