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污染水体中生长的贝类是传播甲型肝炎的重要途径之一。本文介绍了一种抗体捕捉后进行ＰＣＲ扩增来检测贝肉中甲肝病毒（ｈｕｍａｎｈｅｐａｔｉｔｉｓＡｖｉｒｕｓ，ＨＡＶ）的方法。贝肉中ＨＡＶ的浓集回收采用洗脱一沉淀法井加以改进，用脊髓灰质炎病毒作为指示病毒，经二次沉淀后病毒的回收经率为２６％，３０克贝肉中的病毒浓集回收在１．０～１．５ｍｌ上清液中。此方法计算检测限＜３０－３００ＨＡＶ／３ｇ贝肉。从１４份大连海域标本中，用该法检测出阳性标本７份，阳性率为５０％。此浓集病毒的方法还可应用于检测贝肉中污染的脊髓灰质炎病毒、诺瓦克病毒、轮状病毒等其它病毒。
Shellfish that pollutes the water body is one of the important ways to transmit Hepatitis A. This article describes a method for detecting hepatitis A virus (HAV) in shellfish by PCR amplification after antibody capture. The recovery of HAV in shellfish meat was improved by the elution-precipitation method. The poliovirus was used as the indicator virus, and the recovery rate of the virus after secondary sedimentation was 26%. The virus concentration in 30 g shellfish meat It is recovered in 1.0 to 1.5 ml of supernatant. This method calculates the limit of detection <30-300HAV / 3g shell meat. From 14 samples of Dalian sea area, 7 positive samples were detected by this method, the positive rate was 50%. The method of concentrating the virus can also be applied to detect poliovirus, norovirus, rotavirus and other viruses contaminated in shellfish meat.