论文部分内容阅读
背景:胆汁反流性胃炎是由过多的十二指肠胃反流引起的胃黏膜化学性炎症,与萎缩、肠化生等胃黏膜癌前病变有关。细胞周期蛋白D1(cyclin D1)是细胞周期调节因子,研究显示其与胃癌的发生关系密切。目的:探讨cyclin D1在胆汁反流性胃炎和胃癌中的表达及其意义。方法:收集胃癌组织标本、癌旁非癌组织标本、胆汁反流性胃炎组织标本各50例,以50例正常胃黏膜组织标本作为正常对照。观察胆汁反流性胃炎胃黏膜病变情况;以免疫组化法检测cyclin D1表达。结果:胆汁反流性胃炎组肠上皮化生和异型增生的比例显著高于正常对照组(P<0.05)。Cyclin D1阳性率在正常对照组、胆汁反流性胃炎组、癌旁非癌组和胃癌组中逐渐升高,胃癌组显著高于其余三组(P<0.05),胆汁反流性胃炎组显著高于正常对照组(P<0.05),癌旁非癌组与胆汁反流性胃炎组间差异无统计学意义(P>0.05)。结论:Cyclin D1在胃癌的恶性演变过程中发挥重要作用,且可能参与了胆汁反流性胃炎发展为胃癌的过程。
BACKGROUND: Bile regurgitant gastritis is a chemical inflammation of the gastric mucosa caused by excessive duodenogastric reflux, which is associated with precancerous lesions of the gastric mucosa such as atrophy and intestinal metaplasia. Cyclin D1 is a cell cycle regulator, studies have shown that it is closely related to the occurrence of gastric cancer. Objective: To investigate the expression and significance of cyclin D1 in bile reflux gastritis and gastric cancer. Methods: Fifty cases of gastric cancer tissues, adjacent non-cancerous tissues and bile reflux gastritis tissues were collected, and 50 normal gastric mucosa specimens were collected as normal control. To observe the gastric mucosal lesions of bile reflux gastritis and the expression of cyclin D1 by immunohistochemistry. Results: The proportion of intestinal metaplasia and dysplasia in bile regurgitant gastritis group was significantly higher than that in normal control group (P <0.05). The positive rate of Cyclin D1 was gradually increased in normal control group, bile reflux gastritis group, non-cancerous gastric cancer group and gastric cancer group, significantly higher in gastric cancer group than in the other three groups (P <0.05), and significantly higher in bile reflux gastritis group (P <0.05). There was no significant difference between adjacent non-cancerous group and bile reflux gastritis group (P> 0.05). Conclusion: Cyclin D1 plays an important role in the malignant progression of gastric cancer and may be involved in the development of bile reflux gastritis.