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目的探讨调节性T细胞(Treg)活化与人乳头瘤病毒(HPV)16存在状态的关系及其与HPV16整合状态的宫颈鳞癌患者临床病理资料之间的相关性。方法 2012年1-10月在中国医科大学附属盛京医院妇产科,采用多重实时PCR技术对HPV16阳性的17例正常宫颈组织、65例宫颈上皮内瘤变(CIN)、60例宫颈鳞癌(CC)的宫颈脱落细胞标本检测E2和E6基因,通过E2/E6比值法评估HPV16 DNA的体内存在状态。采用免疫组化方法检测相应宫颈组织中叉头状/翅膀状螺旋转录因子(Foxp3)的表达。结果 Foxp3阳性积分光密度值在HPV16游离状态、整合状态宫颈组织中,均与宫颈病变严重程度呈正比,在CC与CINⅢ组比较差异有统计学意义(P<0.05)。在同一病变中,HPV16整合型组Foxp3阳性表达均高于游离型组,在CC组比较差异有统计学意义(t=-2.685,P<0.05)。Foxp3阳性在HPV16整合状态宫颈鳞癌中的表达与FIGO分期、组织学分级及淋巴结转移相关(P<0.05),而与年龄无关(P>0.05)。结论 Treg与HPV存在状态密切相关,Treg在HPV持续感染所致宫颈癌的发生及发展中可能起着重要作用。
Objective To investigate the relationship between the activation of regulatory T cells (Tregs) and the status of human papillomavirus (HPV) 16 and the clinicopathological data of patients with cervical squamous cell carcinoma with integrated status of HPV16. Methods From January to October 2012, 17 cases of normal cervical tissue with HPV16 positive, 65 cases of cervical intraepithelial neoplasia (CIN), 60 cases of squamous cell carcinoma of cervix were diagnosed in Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University. (CC) specimens of cervical exfoliated cells were used to detect E2 and E6 genes, and the presence of HPV16 DNA in vivo was evaluated by the E2 / E6 ratio method. Immunohistochemistry was used to detect the expression of forkhead / wing-like helical transcription factor (Foxp3) in corresponding cervical tissues. Results The positive integral optical density value of Foxp3 in HPV 16 free state and integrated cervical tissue was positively correlated with the severity of cervical lesions. There was significant difference between CC and CIN Ⅲ group (P <0.05). In the same lesion, the positive expression of Foxp3 in HPV16-integrated group was higher than that in free-type group, and there was significant difference in CC group (t = -2.685, P <0.05). The expression of Foxp3 in HPV16 cervical squamous cell carcinoma was correlated with FIGO stage, histological grade and lymph node metastasis (P <0.05), but not with age (P> 0.05). Conclusion Treg is closely related to the status of HPV, Treg may play an important role in the occurrence and development of cervical cancer caused by persistent HPV infection.