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目的探讨WASP家族富含脯氨酸同源蛋白1(WASP-family verprolin-homologous protein 1,WAVE1)及基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)和基质金属蛋白酶-9(MMP-9)在卵巢癌的表达及临床意义。方法采用免疫组化SABC三步法检测47例卵巢癌、30例卵巢良性肿瘤和21例正常卵巢组织中WAVE1、MMP-2、MMP-9的表达,并对卵巢癌组织WAVE1与MMP-2、MMP-9的表达进行相关性分析。结果①WAVE1、MMP-2及MMP-9在卵巢癌中的表达均高于在正常卵巢组织及卵巢良性组织中的表达,差异有统计学意义(P<0.05);Spearman相关分析显示,WAVE1和MMP-2、MMP-9两两表达成正相关(r=0.773,r=0.664,P<0.05)。②WAVE1的表达与卵巢癌的临床分期、组织分化及Ca-125水平有关(P<0.05),与年龄、肿瘤的直径无关(P>0.05)。MMP-2、MMP-9表达与卵巢癌的临床分期有关(P<0.05),与年龄、组织分化、Ca-125水平及肿瘤直径无关(P>0.05)。结论 WAVE1和MMP-2、MMP-9在卵巢癌中呈现高表达,早期联合筛查WAVE1和MMP-2、MMP-9指标对卵巢癌病情演变和预后评估有着重要的临床意义。
Objective To investigate the expression of WASP-rich vermicolin-homologous protein 1 (WAVE1) and matrix metalloproteinases-2 (MMP-2) and MMP- 9) in ovarian cancer and its clinical significance. Methods The expressions of WAVE1, MMP-2 and MMP-9 in 47 cases of ovarian cancer, 30 cases of benign ovarian tumor and 21 cases of normal ovarian tissue were detected by immunohistochemical SABC three-step method. The expressions of WAVE1, MMP-2, MMP-9 expression correlation analysis. Results ① The expressions of WAVE1, MMP-2 and MMP-9 in ovarian cancer were significantly higher than those in normal ovarian tissues and benign ovarian tissues (P <0.05). Spearman correlation analysis showed that WAVE1 and MMP-9 -2, MMP-9 expression was positively correlated (r = 0.773, r = 0.664, P <0.05). ② The expression of WAVE1 was correlated with clinical stage, histological differentiation and Ca-125 level in ovarian cancer (P <0.05), but not with age and tumor diameter (P> 0.05). The expression of MMP-2 and MMP-9 was correlated with the clinical stage of ovarian cancer (P <0.05), but not with age, histological differentiation, Ca-125 level and tumor diameter (P> 0.05). Conclusions WAVE1, MMP-2 and MMP-9 are highly expressed in ovarian cancer. The combination of early detection of WAVE1 and MMP-2 and MMP-9 has important clinical significance in the progression and prognosis of ovarian cancer.