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前期降解试验表明2株能够高效降解阿特拉津的菌株:伯克霍尔德氏菌(Burkholderia sp.)LH-ZY01和丛毛单胞菌(Comamonas sp.)A2混合后降解效果明显提高,因此共降解具有重要的应用价值。为节约生产成本,通过响应面设计,利用2株能够高效降解阿特拉津的菌株,通过一系列显著因素优化设计,最终获得高密度培养上述2株菌的发酵培养基配方(g/L):蔗糖6.72,葡萄糖6.75,豆粕粉6.34,K_2HPO_4·3H_2O 0.6,KH_2PO_42.5,MgSO_4·7H_2O 0.3,NaCl 0.4,酵母粉3,pH 7.0~7.2。通过优化发酵配方和发酵方法,获得发酵液的总菌数为102亿/m L,其中伯克霍尔德氏菌菌数为62亿/m L,丛毛单胞菌菌数为40亿/m L。高密度发酵的优化配方为高效降解阿特拉津复合微生物的生产应用提供了重要依据。
The preliminary degradation experiments showed that the two strains capable of efficiently degrading atrazine were significantly degraded after mixed with Burkholderia sp. LH-ZY01 and Comamonas sp. A2, Therefore, total degradation has important application value. In order to save the cost of production, through the design of the response surface, two strains capable of efficiently degrading atrazine were designed and optimized by a series of significant factors to finally obtain the fermentation medium formula (g / L) : Sucrose 6.72, glucose 6.75, soybean meal 6.34, K 2 HPO 4 · 3H 2 O 0.6, KH 2 PO 42.5, MgSO 4 4.7H 2 O 0.3, NaCl 0.4, yeast powder 3, pH 7.0-7.2. By optimizing the fermentation formula and fermentation method, the total number of bacteria in the fermentation broth was 10.2 billion / m L, of which the number of Burkholderia bacteria was 6.2 billion / m L, the number of Comamonas sp. Was 4 billion / m L. The optimized formula of high-density fermentation provides an important basis for the efficient production of atrazine compound microorganisms.