Efficacy of an Yinyanghuo (Herba Epimedii Brevicornus)-Xianmao(Rhizoma Curculiginis) drug pair in a

• 作 者: LIU Chang LIU Lihong LI Nan XIU Aihui ZHANG Zhixing AI Hao
• 机 构: Department of Gy-necological Ward,The Third Affiliated Hospital,Jinzhou Med-ical University,Jinzhou
• 出 处: 中医杂志（英文版）
• 发表日期: 2021年4期
• 关键词: polycystic ovary syndrome Pharmacol-ogy Yinyanghuo (Herba Epimedii Brevicornus)-

OBJECTIVE:To identify active compounds in an Yinyanghuo (Herba Epimedii Brevicornus)-Xianmao(Rhizoma Curculiginis) drug pair (ECD) and investi-gate its efficacy on polycystic ovary syndrome(PCOS),and its possible mechanism in a rat model of PCOS.METHODS:A network pharmacology approach in-volving a characteristic drug assessment,active compound and target prediction,PCOS gene col-lection as well as network analysis was employed.The ovary morphology after treatment was ob-served using an animal model and western blot-ting and real-time PCR were used to verify AKT1 as the molecular target.RESULTS:Six networks were constructed,an active compound-target network for the ECD (C-T net-work),a drug-target network (D-T network),a re-lated genes network,a targets interaction net-work,a key genes interaction network,and a gene-pathway network.A total of 41 compounds and 261 targets were identified for the ECD,232 PCOS-related genes,31 cogenes,and 14 path-ways.These pathways may be involved in the effi-cacy of ECD on PCOS.The proteins most involved in the signal pathways for all targets were AKT1,IL6,INSR,ESR,and GSK3B.The AKT1 target was selected for experimental verification.Based on the Western blot and real-time PCR results,the ex-pression of AKT1 in the PCOS model varied after treatment with ECD.CONCLUSIONS:Our findings suggest that the ECD can reverse the negative morphological changes in ovarian tissue that occur in model rats of PCOS.AKT1 may be a key mediator of the observed abili-ty of the ECD to protect against PCOS in the model rats.