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目的了解温州市无偿献血者戊型肝炎病毒血症及基因分型的情况。方法对2008年2～10月浙江省温州市中心血站的无偿献血者进行整群抽样,检测抗HEV IgG以及IgM抗体,并对抗HEV IgM阳性的标本进行RT-PCR检测,经测序后分析其基因型及序列同源性。结果3044例无偿献血者中,HEV IgG阳性率为33.28%(1013/3044),IgM阳性率为0.92%(28/3044)。对28例HEV IgM阳性标本进行PCR检测共发现3例核酸阳性,核酸总体阳性率为0.1%,其中2例为基因Ⅳ型感染,1例为基因Ⅰ型感染。结论无偿献血者中存在一定比例的HEV病毒携带者,有必要对现有血液筛查策略下输血途径传播戊肝的风险进行评估。
Objective To understand the situation of hepatitis E viremia and genotyping in non-compensation blood donors in Wenzhou. Methods From February to October in 2008, the blood donors in Wenzhou Blood Center of Zhejiang Province were enrolled by cluster sampling to detect anti-HEV IgG and IgM antibody. RT-PCR was carried out on the positive samples of HEV IgM. After sequencing, Genotype and sequence homology. Results Among 3044 blood donors, the positive rate of HEV IgG was 33.28% (1013/3044) and the positive rate of IgM was 0.92% (28/3044). Three of the 28 HEV IgM positive specimens were detected by PCR. The positive rate of nucleic acid was 0.1%, of which 2 were genotype Ⅳ infection and 1 was genotype Ⅰ infection. Conclusions There is a certain proportion of HEV carriers in the blood donors. It is necessary to evaluate the risk of transmitting HE in the blood transfusion through the existing blood screening strategies.