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目的:利用多波长高效液相色谱法,建立消肿止痛酊中3种有效成分(栀子苷、丹皮酚、樟脑)的含量测定方法,考察该产品在有效期内不同生产日期的内在质量。方法:采用Waters SunFire C18(4.6 mm×250 mm,5μm)色谱柱,柱温30℃;甲醇(A)-水(B)为流动相,以1 mL.min-1流速梯度洗脱;检测波长:238 nm(0～14 min,检测栀子苷)、274 nm(15～32 min,检测丹皮酚)、288 nm(33～60 min,检测樟脑)。结果:在60 min内消肿止痛酊中栀子苷、丹皮酚、樟脑3种有效成分被完全分离,峰面积与其浓度呈良好的线性关系(r=0.9999);加样回收率(n=6):栀子苷为100.9%(RSD=0.6%),丹皮酚为100.2%(RSD=1.4%),樟脑为100.6%(RSD=1.0%)。结论:本方法操作简单,结果准确,为有效控制消肿止痛酊质量,提升质量标准,提供了一种可靠的检测方法。
OBJECTIVE: To establish a method for the determination of three active ingredients (geniposide, paeonol, camphor) in Qudachengtong tincture by multi-wavelength high performance liquid chromatography (HPLC), and to examine the intrinsic quality of the product at different production dates. Methods: A Waters SunFire C18 (4.6 mm × 250 mm, 5 μm) column was used. The column temperature was 30 ℃. The mobile phase was methanol (A) -water (B) : 238 nm (0-14 min, detection of geniposide), 274 nm (15-32 min, detection of paeonol), 288 nm (33-60 min, detection of camphor). Results: The active components of geniposide, paeonol and camphor were completely separated within 60 min, and the peak area was linear with the concentration (r = 0.9999). The recovery rate (n = 6): Geniposide 100.9% (RSD = 0.6%), paeonol 100.2% (RSD = 1.4%) and camphor 100.6% (RSD = 1.0%). Conclusion: The method is easy to operate and the results are accurate. It provides a reliable method to control the quality of relieving pain and relieving pain tincture and improve the quality standard.