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为了掌握蒸汽爆破玉米秸秆的酶解特性,研究了不同底物浓度、酶浓度、温度对反应速率的影响。运用米氏方程对酶解动力学过程进行拟合,结果表明,纤维素酶对该玉米秸秆的水解反应在反应前3 h符合一级反应,可用米氏方程对其进行拟合。在转速为120 r/min、酶浓度为1.2 FPU/mL、pH 5.0、温度为45℃时米氏常数Km为11.71 g/L,最大反应速率Vm为1.5 g/(L.h)。确立了包括底物浓度、酶浓度、温度在内的酶解动力学模型,该模型适合温度为30℃~50℃。
In order to grasp the enzymolysis characteristics of steam explosion corn stalks, the effect of different substrate concentration, enzyme concentration and temperature on the reaction rate was studied. The Mie equation was used to fit the kinetic process of enzymatic hydrolysis. The results showed that the hydrolysis of the corn stover by the cellulase met the first order reaction 3 h before the reaction, and was fitted by the Mie equation. The Mk constant Km was 11.71 g / L and the maximum reaction rate Vm was 1.5 g / (L · h) at the speed of 120 r / min, the enzyme concentration of 1.2 FPU / mL, the pH of 5.0 and the temperature of 45 ℃. The enzymolysis kinetic model including substrate concentration, enzyme concentration and temperature was established. The model was suitable for the temperature from 30 ℃ to 50 ℃.