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目的构建一个结构简单,并有利于细胞贴壁培养的微流控芯片系统。方法利用生物交联剂将RGD肽共价连接在芯片微通道表面,通过与细胞膜表面整合素家族的特异性识别,促进细胞的黏附与贴壁生长;考察不同浓度RGD的功能化效果。结果化学交联法能将RGD有效的固定在芯片微通道表面,形成均匀一致的RGD层。RGD功能化处理的细胞芯片具有良好的生物相容性,可在无CO2的室温条件下实现较长时间的细胞培养。结论表面修饰能实现RGD肽在微流控芯片材料表面的固定并保持其生物活性,有利于细胞在微通道中的贴壁培养。
Objective To construct a microfluidic chip system with simple structure and good cell adhesion culture. Methods RGD peptide was covalently attached to the microchannel surface by biocrosslinker. Cell adhesion and adhesion growth were promoted by specific recognition of integrin family on the surface of cell membrane. Functional effects of RGD at different concentrations were investigated. Results The chemical cross-linking method can effectively fix RGD on the microchannel surface of the chip to form a uniform RGD layer. The RGD-functionalized cell chip has good biocompatibility and can achieve longer cell culture at room temperature without CO2. Conclusion The surface modification can achieve the fixation of RGD peptide on the surface of microfluidic chip material and maintain its biological activity, which is conducive to the adherent culture of cells in the microchannels.