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目的了解牛奶中致泻性大肠杆菌的污染状况及耐药性,为食品安全风险评估和有效控制食源性疾病的发生提供依据。方法对牛奶中的致泻性大肠杆菌进行分离鉴定,并进行血清分型和耐药性分析;药敏试验是利用Sensitire微生物药敏分析系统对分离株进行8种抗生素的敏感性实验。结果在110份牛奶中分离出大肠杆菌59株,检出率为53.6%(59/110);其中27株为致泻性大肠杆菌,检出率为24.5%(27/110),血清型主要包括O128K67、O28K73、O86K61和O78K60,种类以EPEC(16株)和EIEC(6株)为主。在分离的27株致泻性大肠杆菌中,10株具有耐药性,其中2株为单独耐药,8株为多重耐药。结论牛奶中存在致泻性大肠杆菌的污染,且检出的阳性菌株有多重耐药现象。
Objective To understand the status and drug resistance of diarrhea-causing Escherichia coli in milk and provide the basis for food safety risk assessment and effective control of food-borne diseases. Methods The diarrhea-causing Escherichia coli in milk was isolated and identified, and the serotyping and drug resistance analysis were carried out. The susceptibility test was conducted on Sensitire Microbial Susceptibility Assay System for 8 antibiotics. Results 59 isolates of Escherichia coli were isolated from 110 milk samples, with a detection rate of 53.6% (59/110). Of these, 27 were diarrheogenic E. coli with a detection rate of 24.5% (27/110), serotypes Including O128K67, O28K73, O86K61 and O78K60, the type of EPEC (16 strains) and EIEC (6 strains) based. Of 27 isolates of diarrheal Escherichia coli, 10 were resistant, of which 2 were resistant alone and 8 were multidrug-resistant. Conclusion There is diarrhea-causing Escherichia coli in milk, and the positive strains detected are multi-drug resistant.