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目的对海南岛黎族人群血小板-1～6、15抗原系统进行基因分型,探讨血小板抗原分布与随机血小板输注的关系以及血小板抗原分布与疾病的相关性,为黎族人群临床血小板输注提供实验依据。方法采用PCR-SSP方法对180名黎族人进行HPA-1～6、15抗原进行基因分型。结果本研究揭示了海南岛黎族人HPA-1～6、15抗原基因频率分布概况,证实黎族人HPA-2、-3、-5、-6、-15系统具有多态性。结论研究结果提示黎族人在随机血小板输注中,供受者HPA-3系统不配合的机会为37.49%,HPA-15系统不配合的机会为36.93%,是随机血小板输注的关注重点。HPA-5系统不配合的机会为6.23%,HPA-2、-6系统不配合的机会只有0.56%。而HPA-1、-4抗原未检出相应HPA-b基因,同种免疫发生的危险性会非常低。
Objective To investigate the relationship between platelet antigen distribution and random platelet transfusion and the relationship between platelet antigen distribution and disease in the Li population in Hainan Island and provide an experimental basis for clinical platelet transfusion in accordance with. METHODS: 180 Li people were genotyped by HPA-1 ~ 6,15 antigen by PCR-SSP method. Results The frequency distribution of HPA-1 ~ 6,15 antigens in Li people in Hainan Island was revealed in this study. It is confirmed that the HPA-2, -3, -5, -6 and -15 systems in Li people have polymorphism. Conclusions The results suggest that in the randomized platelet transfusion, Li people have 37.49% chance of donating HPA-3 system and 36.93% failing HPA-15 system, which is the focus of random platelet transfusion. HPA-5 system does not match the chance of 6.23%, HPA-2, -6 system does not match the chance of only 0.56%. The HPA-1, -4 antigen did not detect the corresponding HPA-b gene, alloimmune risk will be very low.