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采用营养液水培的方法,研究了外源一氧化氮(Nitric oxide,NO)对50mmol.L-1NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响。结果表明:10~400μmol.L-1NO供体硝普钠(Sodium nitroprusside,SNP)能显著缓解NaCl胁迫对黄瓜植株造成的伤害,100μmol.L-1SNP缓解效果最好,可提高幼苗的生长量,增强幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性,提高了叶片叶绿素和脯氨酸(Pro)含量、净光合速率(Pn)、蒸腾速率(Tr)及气孔导度(Gs);降低了叶片丙二醛(MDA)和过氧化氢(H2O2)的含量、超氧阴离子(O.2-)的产生速率、质膜透性和胞间二氧化碳浓度(Ci)。
The effects of nitric oxide (NO) on the growth, reactive oxygen species metabolism and photosynthetic characteristics of cucumber seedlings under 50mmol.L-1NaCl stress were studied by hydroponics. The results showed that sodium nitroprusside (SNP), a donor of 10-400μmol.L-1NO, could significantly alleviate the damage caused by NaCl stress on cucumber plants. The 100μmol·L-1 SNP had the best alleviation effect, which could increase the seedling growth, The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) in seedling leaves were increased and the content of chlorophyll and proline (Pro) , Net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs), and the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) Production rate, plasma membrane permeability and intercellular carbon dioxide concentration (Ci).