论文部分内容阅读
目的与方法：针对大鼠血栓素合酶基因设计并合成的三条反义寡脱氧核苷酸片段（ＯＤＮⅠ，ＯＤＮⅡ，ＯＤＮⅢ）与体外培养的大鼠腹腔巨噬细胞温育２４ｈ，筛选能有效抑制血栓素合酶及ＴＸＡ２生成的片段。结果：ＯＤＮⅠ组培养基中血栓素Ａ２含量在三个浓度点（４，８，１２μｍｏｌ／Ｌ）（１０４６，９１５，８１５ｐｇ／μｇｃｅｌｐｒｏｔｅｉｎ）均较对照组（１２７０，１２８４，１２１２ｐｇ／μｇｃｅｌｐｒｏｔｅｉｎ低（Ｐ＜０．０１）。ＯＤＮⅡ，ＯＤＮⅢ组培养基中ＴＸＡ２含量与对照组相比无显著差异。细胞ＲＮＡ斑点杂交结果显示：ＯＤＮⅠ组斑点积分光密度较对照组明显降低，而ＯＤＮⅡ，ＯＤＮⅢ组斑点积分光密度与对照组相比无明显差异。结论：ＯＤＮⅠ可抑制血栓素合酶基因表达
Objective and Methods: Three antisense oligodeoxynucleotide fragments (ODNⅠ, ODNⅡ, ODNⅢ) designed and synthesized for rat thromboxane synthase gene were incubated with rat peritoneal macrophages cultured in vitro for 24 h. Screening could effectively inhibit Thromboxane synthase and TXA2-generated fragments. Results: Compared with control group, the content of thromboxane A2 in ODNⅠgroup was significantly higher than that in control group at three concentration points (4,8,12μmol / L) (1046,915, 815pgpg / μgcelprotein) 0,1284,1212pg / μgcelprotein low (P <0.01) .There was no significant difference in TXA2 content between ODNⅡand ODNⅢgroups compared with the control group.The results of dot blot hybridization showed that ODNⅠgroup The optical density was significantly lower than that of the control group, but there was no significant difference between the ODN ¢ ò and ODN ¢ ó group in the optical density of spot integrations.Conclusion: ODN I can inhibit the expression of thromboxane synthase