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人类正常妊娠时母亲虽可接受同种异体胎儿-胎盘的存在,但其免疫系统可产生直接针对胎盘合胞体滋养层决定簇的抗体.经大鼠和兔免疫证实,膜囊表面上有唯一的滋养层特异抗原系统.本文作者用ELSA检测母亲血清中能与膜结合决定簇起反应的IgG和IgM抗体,以证实人正常妊娠及是否产生直接针对胎盘的抗体应答.用人胎盘合胞体滋养层的刷缘制备胎盘胞浆膜囊抗原,经ELISA检测母亲血清,出现阳性反应的最高稀释度为终点滴度.并用免疫单扩散法测定
While normal human pregnancy, although the fetus can accept allogeneic fetus - placenta, but the immune system can produce antibodies directed against placental syncytiotrophoblast determinants.After rat and rabbit immunization confirmed that there is a unique membrane surface Trophoblast-specific antigen system.The authors use ELSA to detect IgG and IgM antibodies that react with the membrane-bound determinants in the maternal serum to confirm normal human pregnancy and whether an antibody response is directed against the placenta. The brush margin was used to prepare placental cyst antigen of placenta, the serum of the mother was detected by ELISA, and the highest dilution of the positive reaction was the final titer.