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目的调查黑龙江省牡丹江口岸地区鼠类巴尔通体感染情况。方法 2014年6月,在牡丹江口岸草地及绿化带利用夹夜法和鼠笼法共捕获鼠类33只,采集其脾样本;采用PCR方法检测鼠类巴尔通体感染情况,将阳性样本进行基因测序鉴定。结果共检出巴尔通体核酸阳性样本6份,感染率为18.18%,其中5份分离自黑线姬鼠,1份分离自褐家鼠。牡丹江口岸至少存在4种基因型巴尔通体,包括Bartonella taylorii、B.phoceensis、对人类有致病性的B.grahamii及未定种巴尔通体,提示牡丹江口岸存在巴尔通体基因型多样性的特征。结论黑龙江省牡丹江口岸鼠类存在巴尔通体感染,且携带人类致病性的巴尔通体,对人群有致病风险,应加强口岸地区的鼠类防控能力。
Objective To investigate the infection of Bartonella rickettsi in Mudanjiang port area of Heilongjiang Province. Methods In June 2014, a total of 33 rodents were collected from the grassland and green belt of Mudanjiang Port by using the method of clip-nocturnal night and squirrel cage to collect splenic samples. The positive samples were sequenced by polymerase chain reaction (PCR) Identification. Results A total of 6 specimens of Bartonella hendersoni were detected positive, with an infection rate of 18.18%. Among them, 5 were isolated from Apodemus agrarius and 1 from Rattus norvegicus. There are at least four genotype Bartonella species in the Mudanjiang port, including Bartonella taylorii, B. phoceensis, B. grahamii which is pathogenic to humans, and Bartonella species which have not been identified, suggesting that Bartonella genotype diversity exists in Mudanjiang port. Conclusions The Bartonella body infected with Bartonella in Mudanjiang Port of Heilongjiang Province and carries human pathogenic Bartonella body has risk of disease to the population, and the prevention and control of rodents in port area should be strengthened.