一氧化氮不介导内毒素引起的离体大鼠脊髓释放降钙素基因相关肽

• 作 者: 王宪 唐跃明 韩启德
• 机 构: 北京医科大学第三医院血管医学研究所
• 出 处: 北京医科大学学报
• 发表日期: 1996年04期
• 关键词: 离体大鼠 神经肽 痛觉过敏 释放过程 预孵育 工具药 兴奋性氨基酸 断头处死 兔血清 文献报道

目的：探讨一氧化氮（ＮＯ）及ＮＯ合成酶（ＮＯＳ）抑制剂对内毒素引起的大鼠脊髓降钙素基因相关肽（ＣＧＲＰ）释放的影响。方法：采用离体大鼠脊髓切片孵育，以放射免疫法测定ＣＧＲＰ的释放。结果：ＮＯＳ的合成底物Ｌ－Ａｒｇ；ＮＯ供体ＳＮＡＰ和ＳＮＰ；各种ＮＯＳ抑制剂Ｌ－ＮＮＡ，Ｌ－ＮＡＭＥ，Ｌ－ＮＭＭＡ和７－ＮＩ对离体大鼠脊髓ＣＧＲＰ的基础释放以及内毒素引起的ＣＧＲＰ释放均无明显影响。结论：ＮＯ在离体脊髓水平没有参与内毒素引起的ＣＧＲＰ释放过程。

Objective: To investigate the effects of nitric oxide (NO) and NO synthase (NOS) inhibitors on endotoxin-induced release of calcitonin gene-related peptide (CGRP) in rat spinal cord. Methods: The isolated rat spinal cord sections were incubated and the release of CGRP was measured by radioimmunoassay. RESULTS: The basic release of CGRP from the spinal cord of isolated rat as well as the release of N-ascorbic acid (L-Arg), NO donor SNAP and SNP, various NOS inhibitors L-NNA, L-NAME, L-NMMA and 7-NI Toxins induced CGRP release had no significant effect. CONCLUSION: NO is not involved in endotoxin-induced release of CGRP from isolated spinal cord.