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1植物名称密花胡颓子(Elaeagnus conferta Roxb.),又名羊奶果。2材料类别茎段。3培养条件(1)种子萌发培养基:MS;(2)不定芽诱导培养基:MS+BA 3.0 mg·L~(-1)(单位下同)+NAA 0.1;(3)增殖培养基:MS+BA 2.0+NAA 0.1;(4)壮苗培养基:MS+BA 0.5+NAA 0.1;(5)生根培养基:1/2MS+IBA 2.0+NAA 0.1。上述培养基中均添加30 g·L~(-1)蔗糖和6.5 mg·L~(-1)卡拉胶,pH 5.8。培养温度为(25±2)℃,光照强度为40μmo1·m~(-2)·s~(-1),光照时间为
1 plant name dense flowers Elaeagnus conferta Roxb., Also known as sheep milk fruit. 2 material category stem segment. 3 Culture conditions (1) Seed germination medium: MS; (2) Adventitious bud induction medium: MS + BA 3.0 mg · L -1 (unit same below) + NAA 0.1; MS + BA 2.0 + NAA 0.1; (4) strong seedling medium: MS + BA 0.5 + NAA 0.1; (5) rooting medium: 1/2 MS + IBA 2.0 + NAA 0.1. The medium was supplemented with 30 g · L -1 sucrose and 6.5 mg · L -1 carrageenan at a pH of 5.8. The incubation temperature was (25 ± 2) ℃, the light intensity was 40μmol · m -2 · s -1, the illumination time was