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本实验采用结扎大鼠冠脉左前降支法造成心肌梗塞(MI)的动物模型,旨在观察 MI 急性期同一大鼠梗塞区和非梗塞区氧自由基引起的膜脂质过氧化反应(LPO)的时相变化。LPO 的激活程度以 LPO 的产物丙二醛(MDA)的含量及抗氧化酶超氧化物岐化酶(SOD)的活性变化作为观察指标。取材时间分别为手术后0.75、1.50,3.0、72.0小时。并在3小时实验动物组分别给予天然植物抗氧化剂 Z和 R,观察它们对 LPO 的抑制作用。实验结果表明,心肌梗塞区 MDA 呈双向时相变化,手术后3小时达到高峰。SOD 呈下降趋势。在0.75～3小时 MDA 含量升高与SOD 活性下降呈高度负相关。同时观察到所给予的天然植物抗氧化剂 Z 和 R 对 LPO有较好的抑制作用。
In this study, an animal model of myocardial infarction (MI) induced by ligation of the left anterior descending coronary artery in rats was used to observe lipid peroxidation (OPO) induced by oxygen free radicals in the same infarcted and non-infarcted areas of rats in the acute phase of MI. ) Phase changes. The activation of LPO was measured by the content of malondialdehyde (MDA), the product of LPO, and the activity of the antioxidant enzyme superoxide dismutase (SOD). The time of drawing was 0.75, 1.50, 3.0 and 72.0 hours after surgery. The natural plant antioxidants Z and R were respectively given in the experimental animal group for 3 hours to observe their inhibitory effect on LPO. The experimental results showed that the MDA in the myocardial infarction area showed a biphasic phase change and peaked at 3 hours after surgery. SOD showed a downward trend. At 0.75 to 3 hours, elevated MDA content was highly negatively correlated with decreased SOD activity. At the same time, it was observed that the natural plant antioxidants Z and R had a good inhibitory effect on LPO.