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研究人类白血病细胞株Ｋ５６２及耐长春新碱的Ｋ５６２变异株Ｋ５６２／ＶＣＲ对长春新碱诱导凋亡的敏感性的差异，以探讨抗凋亡在白血病多药耐药性（ＭＤＲ）中的作用。方法将Ｋ５６２及Ｋ５６２／ＶＣＲ分别用浓度为０、０．００１、０．０１０μｇ／ｍｌ的长春新碱诱导２４ｈ，用流式细胞仪ＰＩ染色法、ＴＵＮＥＬ法及ＥＬＩＳＡ法分别进行细胞凋亡检测。结果经浓度为０、０．００１μｇ／ｍｌ长春新碱诱导后，Ｋ５６２细胞凋亡率分别为（４．１０±０．１７）％、（１３．３０±３．７４）％，而Ｋ５６２／ＶＣＲ细胞凋亡率分别为（３．６１±０．６９）％、（９．０６±４．２４）％，统计学显示两者差异无显著性（ｎ＝３，Ｐ＞０．０５）。而长春新碱浓度达０．０１０μｇ／ｍｌ时，Ｋ５６２及Ｋ５６２／ＶＣＲ细胞凋亡率分别为（３６．４８±６．７０）％、（１０．４５±３．７１）％，两者差异有极显著意义（ｎ＝３，Ｐ＜０．０１）。ＴＵＮＥＬ及ＥＬＩＳＡ检测结果与此类似。结论Ｋ５６２及Ｋ５６２／ＶＣＲ对长春新碱诱导细胞凋亡的敏感性不同，耐药细胞对药物诱导细胞凋亡的抵抗性增强可能在白血病ＭＤＲ发生中有重要作用。
The sensitivity of human leukemia cell line K562 and K562/VCR resistant to vincristine to vincristine-induced apoptosis was studied to investigate the role of anti-apoptosis in multidrug resistance (MDR) of leukemia. Methods K562 and K562/VCR were induced with vincristine at concentrations of 0, 0.001, and 0.010 μg/ml respectively for 24 hours. Cell apoptosis was detected by flow cytometry PI staining, TUNEL assay, and ELISA. Results The apoptosis rate of K562 cells was (4.10±0.17)% and (13.30±3.74)%, respectively, after induction with vincristine at a concentration of 0, 0.001 μg/ml, while K562/VCR The apoptosis rate was (3.61±0.69)% and (9.06±4.24)%, respectively. Statistically, there was no significant difference between the two groups (n=3, P>0.05). When the vincristine concentration reached 0.010μg/ml, the apoptotic rates of K562 and K562/VCR cells were (36.48±6.70)% and (10.45±3.71)%, respectively. Very significant (n=3, P<0.01). TUNEL and ELISA test results are similar. Conclusion The sensitivity of K562 and K562/VCR to vincristine-induced apoptosis is different. The enhanced resistance of drug-resistant cells to drug-induced apoptosis may play an important role in the development of MDR in leukemia.