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目的探讨并比较缺血预处理与后处理对肾脏的缺血/再灌注损伤的保护作用。方法将30只雄性SD大鼠随机分为4组:假手术组(Sham)、模型对照组(I/R)、缺血预处理组(I-Precon组)、缺血后处理组(I-Postcon组)。后3组均切除右侧肾脏,左肾缺血60min,但I-Precon组在缺血60min前先将左肾缺血5min,再通血2min,循环3次后进行,I-Postcon组在缺血60min后再进行开放10s+阻断10s循环处理6次。各组处理之后,均再灌注4h。实验结束后检测血浆尿素氮(BUN)、肌酐(Cr)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量,HE染色观察各组肾组织病理变化。结果 I-Precon组和I-Postcon组与I/R组比较,大鼠血浆BUN、Cr、MDA含量明显降低(P<0.05),SOD活性明显升高(P<0.05);形态学结果显示,I-Precon组和I-Postcon组大鼠肾损伤程度明显低于I/R组,且I-Postcon组肾损伤轻于I-Precon组。结论缺血预处理与后处理均对大鼠肾脏缺血/再灌注损伤有保护作用,其机制可能与抗氧化作用有关。
Objective To investigate and compare the protective effect of ischemic preconditioning and postconditioning on renal ischemia / reperfusion injury. Methods Thirty male Sprague-Dawley rats were randomly divided into 4 groups: Sham, I / R group, I-Precon group, I- Postcon group). The left kidney was excised 60min after the left kidney was removed in all three groups. However, in the I-Precon group, the left kidney was ischemia 5min, then the blood 2min, and the circulation 3 times before the ischemia 60min. Blood 60min and then open 10s + block 10s cycle 6 times. After each treatment, reperfusion 4h. After the experiment, the contents of BUN, Cr, MDA and SOD were measured. The pathological changes of kidney were observed by HE staining. Results The levels of BUN, Cr and MDA were significantly decreased (P <0.05) and the activity of SOD was significantly increased in I-Precon group and I-Postcon group compared with I / R group (P <0.05) Kidney injury in I-Precon group and I-Postcon group was significantly lower than that in I / R group, and I-Postcon group was lighter than I-Precon group. Conclusions Both ischemic preconditioning and postconditioning have a protective effect on renal ischemia-reperfusion injury in rats, which may be related to the anti-oxidative effect.