论文部分内容阅读
以具有生物相容性的羧基甜菜碱甲基丙烯酸酯(CBMA)为单体、过硫酸铵为引发剂、亚硫酸钠为还原剂,采用自由基聚合工艺制备了羧基甜菜碱两性离子聚合物PCBMA,并对其进行核磁氢谱和凝胶渗透色谱表征,结果显示聚合物具有预期结构。进一步以葡萄糖氧化酶(GOD)为模型酶,分别制备了添加和未添加聚合物PCBMA的GOD溶液,在四种不同温度下探究酶的活性与时间的关系。结果表明,添加PCBMA的GOD在65℃条件下经过12h后,其活性浓度是对照样GOD 1h时活性浓度的103%;未添加PCBMA的对照样12h后GOD活性浓度仅为1h时的35%。羧基甜菜碱两性离子聚合物能够有效地维持酶的活性,并且温度越高聚合物对酶活性维持的效果越明显。
Using carboxymethyl betaine methacrylate (CBMA) as monomer, ammonium persulfate as initiator and sodium sulfite as reducing agent, carboxyl betaine zwitterionic polymer PCBMA was prepared by free radical polymerization The results of 1H NMR and gel permeation chromatography show that the polymer has the expected structure. Further, glucose oxidase (GOD) was used as model enzyme to prepare GOD solution with and without polymer PCBMA respectively. The relationship between enzyme activity and time was explored at four different temperatures. The results showed that the activity of GOD with PCBMA added was 103% of that of control GOD at 12h after 12h at 65 ℃. The GOD activity of PCBMA-free GOD after 12h was only 35% at 1h. Carboxyl betaine zwitterionic polymers are effective at maintaining enzyme activity, and at higher temperatures the effect of the polymer on the maintenance of enzyme activity is more pronounced.