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目的:比较不同提取方法对刺五加叶中挥发性化学成分的得率,并对其挥发性化学成分进行分析。方法:采用同时蒸馏萃取法(SDE)与常规提取法(水蒸气蒸馏法、溶剂法)对刺五加叶药材挥发性化学成分的提取收率进行比较研究,用GC/MS法对其挥发性化学成分进行分离鉴定,用峰面积归一化法得出各化学成分在挥发性组分中的相对百分含量。结果:共分离出51个成分,确认了其中39种化学成分,主要为萜类及其含氧衍生物(68.85%)和芳香族类化合物(6.32%),其中,(+)匙-叶桉油烯醇含量最高,达19.89%,其余依次为1甲-基-5亚-甲基-8-[1甲-基乙基]-1,6环-癸二烯(7.02%)和1甲-基-1乙-烯基-2,4二-异丙烯基-环己烷(5.59%)等。结论:同时蒸馏萃取法是提取刺五加叶挥发性物质的优良方法。采用GC-MS法可以高效、适用、准确地分析、鉴定药材中挥发性物质的组成,对深入探索中药的药用价值具有积极的促进作用。
OBJECTIVE: To compare the yield of volatile chemical constituents in the leaves of Acanthopanax senticosus with different extraction methods, and analyze their volatile chemical constituents. Methods: The extraction yield of volatile chemical constituents from the leaves of Acanthopanax senticosus L. was compared by simultaneous distillation extraction (SDE) and conventional extraction (steam distillation, solvent method), and the volatile compounds were extracted by GC / MS Chemical composition of the separation and identification, peak area normalization method to draw the chemical composition of the relative content of volatile components. Results: A total of 51 constituents were isolated and 39 chemical constituents were identified, mainly terpenoids and their oxygen-containing derivatives (68.85%) and aromatic compounds (6.32%), of which, The highest content of oleanols was 19.89%, and the others were 1-methyl-5-methyl-8- [1 -methylethyl] -1,6-cyclopentadiene (7.02% -yl-1-en-2,4-di-isopropenyl-cyclohexane (5.59%) and the like. Conclusion: Simultaneous distillation extraction is an excellent method for extracting volatiles from Acanthopanax senticosus. The GC-MS method can effectively, conveniently and accurately analyze and identify the composition of volatile compounds in medicinal materials, which has a positive effect on further exploring the medicinal value of traditional Chinese medicine.