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猪流行性腹泻病毒(PEDV)野毒株在Vero细胞中连续传93代后减毒,并被命名为KPEDV-9,使用聚合酶链反应技术对其M基因进行克隆并测序后发现,弱毒株在核昔酸序列和氨基酸序列上与传代前毒株分别有98.97%和98.24%的同源性。用KPEDV-9弱毒?
The porcine epidemic diarrhea virus (PEDV) wild-type strain was continuously attenuated in Vero cells for 93 passages and was named KPEDV-9. The M gene was cloned and sequenced using polymerase chain reaction (PCR) There were 98.97% and 98.24% homology with the pre-passage virus in the nucleotide sequence and the amino acid sequence respectively. KPEDV-9 with poison?