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AIM:To study the metabolic profile of human umbilical mesenchymal stem cells(HUMSC) and adipogenic differentiation by nuclear magnetic resonance(NMR) spectroscopy.METHODS:HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone,3-isobutyl-1-methylxanthine,indomethacin,and insulin to the culture medium.Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction.Perchloric acid extracts of the HUMSCs and adipocytes(about 7 ×10 6) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy.RESULTS:Several major metabolites,such as:choline,creatine,glutamate and myo-inositol,acetate,and some fatty acids/triglycerides,were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation.HUMSCs are characterized by an unusually low number of NMR-detectable metabolites,high choline,acetate,glutamate and creatine content.However,the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids,and non-detectable creatine.CONCLUSION:The biomarkers of HUMSCS and adipocytes were obtained and assigned.NMR spectroscopy will be a promising tool for monitoring stem cell differentiation.
AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy. METHODS: HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone, 3- isobutyl-1-methylxanthine, indomethacin, and insulin to the culture medium. Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction. Perchloric acid extracts of the HUMSCs and adipocytes (about 7 × 10 6) were characterized for metabolites by using in vitro high resolution 9.4 T NMR spectroscopy .RESULTS: Several major metabolites, such as: choline, creatine, glutamate and myo-inositol, acetate, and some fatty acids / triglycerides, were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation.HUMSCs are characterized by an unusually low number of NMR-detectable metabolites, high choline, acetate, glutamate and creatine content. However, the metabolic pro files of adipogenic differentiation demonstrated emerged higher methionine and fatty acids, and non-detectable creatine. CONCLUSION: The biomarkers of HUMSCS and adipocytes were obtained and assigned. NMR spectroscopy will be promising tools for monitoring stem cell differentiation.