纤维素酶降解人参细胞胞壁产生的激发子诱导人参培养细胞的反应

• 作 者: 殷俊 刘超 管培珠 蔡伟明
• 机 构: 中国科学院上海植物生理研究所,中国科学院上海植物生理研究所,中国科学院上海植物生理研究所,中国科学院上海植物生理研究所 上海 200032,上海 200032,上海 200032,上海 200032
• 出 处: 实验生物学报
• 发表日期: 1999年03期
• 关键词: 激发子 拟脂类圆球体 愈伤组织 酶活力 苯丙氨酸解氨酶 酚氧化酶 酶降解 脂类代谢 真菌侵染 造粉体

用纤维素酶降解人参细胞壁制备的激发子刺激人参细胞后,电子显微镜的观察结果显示激发子处理诱导了细胞内淀粉粒的降解,出现了细胞内动员能量物质的应急反应;同时还出现了大量拟脂类圆球体。进一步的研究表明,涉及降解淀粉的淀粉酶活力是升高了,而降解脂类物质的酯酶活力是降低的;同时细胞中可溶性糖和总类脂含量是增加的。这些都说明了人参细胞经激发子处理后能量物质的动员以及脂类代谢途径的调整。另外,适当浓度激发子的处理会诱导人参细胞内人参皂苷含量和苯丙氨酸解氨酶活力的升高,而过氧化物酶和多酚氧化酶活力是下降的。

After the ginseng cells were stimulated by the degradation of ginseng cell wall with cellulase, the observation by electron microscopy showed that the elicitor treatment induced the degradation of the intragranular starch granules and the emergent response of intracellular mobilization of energy substances. At the same time, Pseudo-fat sphere. Further studies showed that the amylase activity involved in degrading starch was increased while that of degraded lipids was decreased; at the same time, soluble sugar and total lipid content were increased in the cells. All of these illustrate the mobilization of energy substances by ginseng cells and the regulation of lipid metabolism. In addition, the treatment with the appropriate concentration of elicitor induces an increase in ginsenoside content and phenylalanine ammonia lyase activity in ginseng cells, while the peroxidase and polyphenol oxidase activities are decreased.