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目的 探讨P糖蛋白 (P -gp)对MCF - 7/Adr肿瘤细胞线粒体膜电位的影响。方法 运用流式细胞仪检测P -gp功能细胞抑制组 (用维拉帕米抑制P -gp功能 )和对照组细胞线粒体膜电位 (mitochondrialmembranepotenial,ΔΨm)。结果 P -gp功能抑制组细胞ΔΨm平均荧光强度值是 2 2 .2 5± 2 .36 ;对照组是 2 9.6 6± 2 .38。抑制ΔΨm平均荧光强度值明显低于对照组(P <0 .0 1 )。结论 抑制P -gp功能下调了MCF - 7/Adr细胞线粒体膜电位。
Objective To investigate the effect of P-gp on the mitochondrial membrane potential of MCF - 7 / Adr tumor cells. Methods Flow cytometry was used to detect the mitochondrial membrane potential (ΔΨm) in P-gp functional cell inhibition group (verapamil inhibited P-gp function) and control group. Results The mean fluorescence intensity of ΔΨm in P-gp inhibition group was 22.5 ± 2.36 and in control group was 29.6 ± 2.38. The average fluorescence intensity of ΔΨm was significantly lower than that of the control group (P <0.01). Conclusion Inhibition of P-gp function down-regulated the mitochondrial membrane potential of MCF - 7 / Adr cells.