论文部分内容阅读
目的 探讨钙硒2种营养素联合应用对铅暴露小鼠学习记忆能力的影响.方法 3周龄清洁级昆明小鼠36只,随机分为3组:对照组,铅染毒组.钙硒联合干预组;在实验第20 d进行水迷宫实验,取小鼠的血、脑测定铅含量及NO含量、一氧化氮合酶(NOS)和诱导型一氧化氮合酶(iNOS)活性.结果 小鼠钙硒联合干预组血铅(469.59±58.31)μg/L和脑铅(0.460±0.034)μg/g含量高于对照组,低于铅染毒(885.02±105.77)μg/L和(1.290±0.270)μg/g组,差异有统计学意义(P<0.01);钙硒联合干预组脑NO含量为(8.29±1.22)μmol/g、NOS和iNOS活性分别为(4.39±0.98),(1.65±0.28),均低于对照组(10.27±2.27)ixmoVg,(6.22±1.25),(1.91±0.44)高于铅染毒组(5.66±1.04)μmol/g,(2.66±0.59),(0.67±0.14)差异有统计学意义(P<0.01);对照组、钙硒联合干预组小鼠水迷宫逃避潜伏期分别为(43.8±8.9),(44.1±11.7)s,短于铅染毒组的(85.9±23.5)s,差异有统计学意义(P<0.01),钙硒联合干预组与对照组差异无统计学意义.结论 钙和硒联合应用能有效地促进铅模型小鼠拮抗体内铅的毒性作用.“,”Objective To explore the combined effect of calcium and selenium on learning and memory of mice with lead exposure.Methods Totally 36 three weeks weaning Kunming mice (clean level) were randomly divided into three groups :control,lead group, calcium and selenium combined group.Twenty days after the administration of the exposure, the water labyrinth test was conducted.The contents of lead, nitric oxide (NO) and the activites of nitric oxide synthase (NOS) ,inducible nitric oxide synthae (iNOS) in blood and brain tissues of the mice were also detected.Results The blood lead level of the calcium and selenium combination group(469.59 ± 58.31 μg/L) was lower than that of the lead group and higher than that of control group with significant difference (P <0.01).The cerebral NO content (8.29 ± 1.22 μmoL/g) ,NOS (4.39±0.98) and iNOS activity(1.65±0.28) of calcium and selenium combination group were lower than that of control group and higher than that of the lead group and the difference was statistically significant (P < 0.01).The times of incubation period of water labyrinth of control group (43.8 ± 8.9s), calcium and selenium combination group (44.1 ± 11.7 s) were shorter than that of the lead group with statistically significant difference (P <0.01).Conclusion The combined application of calcium and selenium can effectively resist adverse effect of lead exposure in mice.