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目的探讨金属硫蛋白(m etalloth ione ins,MT)各功能亚型在喉癌组织中的表达与喉癌临床病理生物学行为的关系。方法应用半定量RT-PCR方法,从分子水平研究MT各功能亚型在48例喉癌组织和15例正常黏膜组织的表达,分析MT各功能亚型与喉癌临床病理生物学行为的关系。结果48例喉癌组织中,在43例标本中MT-1 f、MT-1h和MT-2 a在喉癌组织显著高于12例正常组织中的表达(P<0.01),余8例为阴性表达,并且MT-1 f和MT-2 a在中、低分化喉癌组织的表达显著高于高分化组织(P<0.05),而MT-1 a在喉癌组织中表达减少(P<0.05),MT-1x在喉癌组织未见表达,但在正常组织中可见表达。结论MT-1 f和MT-2 a与喉癌分化程度显著相关,而MT-1 a可能与肿瘤细胞的凋亡有关。金属硫蛋白可能参与了喉癌细胞的增殖和凋亡,在喉癌的发生发展过程中起一定的作用。
Objective To investigate the relationship between the expression of various functional subtypes of Metallothionein (MT) in laryngeal carcinoma and the clinicopathologic behavior of laryngeal carcinoma. Methods The expression of MT functional subtypes in 48 cases of laryngeal carcinoma and 15 cases of normal mucosa was studied by semi-quantitative RT-PCR at molecular level. The relationship between MT functional subtypes and clinicopathological behavior of laryngeal carcinoma was analyzed. Results The expression of MT-1 f, MT-1 h and MT-2 a in laryngeal carcinoma was significantly higher than that in 12 normal tissues (P <0.01) in 48 specimens of laryngeal carcinoma, the remaining 8 specimens were The expression of MT-1 f and MT-2 a in moderate and poorly differentiated laryngeal squamous cell carcinoma tissues was significantly higher than that in well-differentiated tissues (P <0.05), while the expression of MT-1 a was decreased in laryngeal squamous cell carcinoma tissues (P < 0.05). MT-Ix was not expressed in laryngeal cancer tissues, but was found in normal tissues. Conclusion MT-1 f and MT-2 a are significantly correlated with the degree of differentiation of laryngeal carcinoma, while MT-1a may be related to the apoptosis of tumor cells. Metallothionein may be involved in the proliferation and apoptosis of laryngeal carcinoma cells, play a role in the occurrence and development of laryngeal carcinoma.