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[目的]探讨Pyrin重组蛋白对博来霉素诱导的小鼠肺纤维化NF-κB信号通路的作用.[方法]选择雌性清洁级BABL/c小鼠,随机分为正常对照组、肺纤维化模型组及Pyrin重组蛋白治疗组.第14 d给小鼠施行全身麻醉取右肺组织行HE及Masson三色染色.用Western blot检测肺组织中NF-κB p65蛋白的表达.[结果]与正常对照组比较,肺纤维化模型组小鼠肺组织中NF-κB p65蛋白表达水平显著升高(P<0.01);与肺纤维化模型组比较,Pyrin重组蛋白治疗组肺组织中NF-κB p65蛋白水平显著降低(P<0.05).[结论]Pyrin重组蛋白可抑制肺纤维化的发生,其机制可能是通过阻断NF-κB信号通路实现的.
[Objective] To investigate the effect of Pyrin recombinant protein on bleomycin-induced pulmonary NF-κB signaling pathway in mice. [Methods] Female BABL / c mice were randomly divided into normal control group, pulmonary fibrosis Model group and Pyrin recombinant protein treatment group.The rats were subjected to general anesthesia on the 14th day to take right lung tissue HE staining and Masson trichrome staining.Western blot was used to detect the expression of NF-κB p65 protein in lung tissue.Results Compared with normal Compared with the model group, the expression of NF-κB p65 protein in the lung tissue of the lung fibrosis model group was significantly increased (P <0.01). Compared with the model group, the expression of NF-κB p65 (P <0.05). [Conclusion] Pyrin recombinant protein can inhibit the occurrence of pulmonary fibrosis, and its mechanism may be through blocking the NF-κB signaling pathway.