论文部分内容阅读
本文应用琼脂平板稀释法测定氧哌嗪青霉素对40株常见致病菌的最低抑菌浓度(MI-C),并应用AVANTAGE分析仪对胆道感染致病菌对氧哌嗪青霉素的敏感性进行测定。结果表明,氧哌嗪青霉素对G~+球菌的MIC为0.5～32μg/ml,对G~-杆菌的MIC为<0.0625～64μg/ml,对胆道常见致病菌的敏感率为75～100%。同时应用AVANTAGE分析仪自动化比浊法探讨了氧哌嗪青霉素胆汁浓度的测定方法。以吸收度(y)对浓度(x)回归,得回归方程为y=0.1878-0.002776x,R=-0.9975。表明在标准浓度2.5～40μg/ml范围内,氧哌嗪青霉素胆汁浓度与吸收度具有良好的线性关系。在三个浓度水平上回收率分别为98.4%、101.3%和105.0%。管间变异<5.7%、日内变异<5.6%、日间变异<6.2%,表明该测定方法较为精确,可以接受并进一步用于临床研究。
In this paper, the minimum inhibitory concentration (MI-C) of piperacillin against 40 common pathogens was determined by agar plate dilution method and the sensitivity of penicillin-susceptible pathogenic bacteria to biliary tract infection was determined by AVANTAGE analyzer . The results showed that MIC for penicillin was 0.5 ~ 32μg / ml for G ~ + cocci and MIC was 0.0625 ~ 64μg / ml for G ~ (-) bacillus. The susceptibility to common pathogens of biliary tract was 75 ~ 100% . At the same time, AVANTAGE analyzer automated turbidimetric method was used to determine the concentration of piperizine penicillin. The regression equation was y = 0.1878-0.002776x and R = -0.9975 with the absorbance (y) of concentration (x). Show that in the standard concentration of 2.5 ~ 40μg / ml range, piperacillin bile concentration and absorption has a good linear relationship. The recoveries at three concentration levels were 98.4%, 101.3% and 105.0%, respectively. Inter-tube variation <5.7%, intra-day variation <5.6%, intra-day variation <6.2%, indicating that the assay is more accurate, acceptable and further used in clinical studies.