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目的研究氟化钠对大鼠睾丸支持细胞氧化应激和凋亡水平的影响。方法采用原代细胞培养方法,将大鼠睾丸支持细胞暴露于0、6、12和24μg/ml的氟化钠中,24 h后检测支持细胞内活性氧(ROS)、丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性,线粒体膜电位及凋亡率水平。结果氟可降低睾丸支持细胞活力(P<0.01);与对照组相比,各氟化钠处理组的ROS水平均显著上升(P<0.01),12和24μg/ml氟化钠组的MDA含量显著上升(P<0.01)。同时,各氟化钠处理组的SOD活性均显著低于对照组(P<0.05或P<0.01)。随着氟化钠剂量的增高,各氟化钠处理组的线粒体膜电位均显著下降,细胞早期凋亡率均显著升高(P<0.01)。结论氟可致睾丸支持细胞氧化应激水平及凋亡率升高。
Objective To investigate the effect of sodium fluoride on oxidative stress and apoptosis in rat testicular sertoli cells. Methods Primary cultured rat cells were exposed to 0, 6, 12 and 24μg / ml sodium fluoride for 24 hours, then the levels of reactive oxygen species (ROS), malondialdehyde (MDA) Content, superoxide dismutase (SOD) activity, mitochondrial membrane potential and apoptosis rate. Results Fluoride decreased the viability of testicular sertoli cells (P <0.01). Compared with the control group, the levels of ROS in each sodium fluoride treatment group increased significantly (P <0.01), while the MDA content in 12 and 24 μg / ml sodium fluoride treatment groups Significantly increased (P <0.01). At the same time, SOD activity in each sodium fluoride treatment group was significantly lower than that in the control group (P <0.05 or P <0.01). With the increase of sodium fluoride dose, the mitochondrial membrane potential of each sodium fluoride treatment group decreased significantly, and the early apoptosis rate of cells increased significantly (P <0.01). Conclusion Fluoride induced oxidative stress in testicular support cells and apoptosis.