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为了探讨糖芯片在沙门氏菌检测上的应用,以沙门氏菌ATCC31685为主要研究对象,制作不同类型的糖芯片用于优化糖芯片检测条件;在优化条件的基础上,降低糖浓度或细菌浓度用于确定糖芯片检测限值;继而系统地研究了糖芯片在沙门氏菌和凝集素FimH拮抗剂筛选上的应用,并探究糖芯片对菌株的破坏性。由实验现象可知,沙门氏菌可与甘露糖化合物修饰的糖芯片结合,其中,以Man-1与NHS玻片所构建的糖芯片效果最优,并且两种不同的沙门氏菌(ATCC31685和ATCC9184)与糖芯片的结合能力不同,因此糖芯片可作为沙门氏菌的一种快速检测手段;在糖浓度为313 mmol/L或细菌浓度106个/mL时荧光信号接近背景值,并且糖芯片检测后对菌株无破坏性,可继续其他检测项目;此外,实验结果表明糖芯片可作为FimH蛋白拮抗剂的筛选工具。
In order to explore the application of sugar chip in Salmonella detection, Salmonella ATCC31685 was selected as the main research object to make different types of sugar chips for optimizing the conditions of sugar chip detection. Based on the optimized conditions, the sugar concentration or bacterial concentration was reduced to determine the sugar Chip detection limit; Then the application of sugar chip in the screening of Salmonella and lectin FimH antagonists was systematically studied, and the destructiveness of the sugar chip to the strain was explored. From the experimental results, it can be seen that Salmonella can be combined with a mannose compound modified sugar chip, wherein the sugar chip constructed by Man-1 and NHS slide has the best effect, and two different Salmonella (ATCC31685 and ATCC9184) So the sugar chip can be used as a rapid detection means of Salmonella; when the sugar concentration is 313 mmol / L or the bacterial concentration is 106 cells / mL, the fluorescence signal is close to the background value, and the strain is not destructive after the detection of the sugar chip , Can continue other tests; In addition, the experimental results show that sugar chip FimH protein antagonists can be used as a screening tool.