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以切花月季‘Samantha’为试材,明确了外层花瓣主要在花朵开放前期扩展。从花瓣中克隆鉴定了3个具有α亚族扩张素保守特征域的基因:RhEXPA5、RhEXPA6和RhEXPA7。在月季花朵1~6级的开放过程中,3个扩张素蛋白基因都有表达,其中RhEXPA7的表达与花瓣快速扩展密切关联;在2级花朵花瓣中RhEXPA5和RhEXPA6的表达几乎不受乙烯影响,而RhEXPA7的表达被乙烯显著抑制。进一步利用35S启动子在拟南芥中过表达RhEXPA7,采用外源ACC处理可明显增加转基因植株幼苗的根毛密度;转RhEXPA7基因种子萌发对ABA的敏感性明显降低,而对NaCl的胁迫耐性明显增加。RhEXPA7与月季花朵开放中花瓣快速扩展进程密切关联,参与了乙烯信号的响应,并可以提高过表达拟南芥的胁迫耐性。
Taking cut rose ’Samantha’ as test material, it was clarified that the outer petal mainly expanded in early stage of flower opening. Cloning from the petals identified three genes with the alpha-subfamily dilator conserved domains: RhEXPA5, RhEXPA6 and RhEXPA7. During the opening of rose flowers from 1 to 6, all three expandin genes were expressed. The expression of RhEXPA7 was closely related to the rapid expansion of petals. The expression of RhEXPA5 and RhEXPA6 in petals of Grade 2 was almost unaffected by ethylene, The expression of RhEXPA7 was significantly inhibited by ethylene. Further, the 35S promoter was used to overexpress RhEXPA7 in Arabidopsis thaliana, and the exogenous ACC treatment could significantly increase the root hair density of transgenic plants. The germination of RhEXPA7 gene significantly reduced the sensitivity to ABA and the stress tolerance to NaCl . RhEXPA7 is closely related to the rapid expansion of petals during the opening of rose flowers, which is involved in the response of ethylene signals and enhances the stress tolerance of overexpression Arabidopsis thaliana.