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目的 :研究维生素B2 在玻碳电极表面吸附的电化学行为及定量分析方法 .方法 :玻碳电极在 8.0mol/LHNO3 介质中 ,于 + 0 .6～ + 2 .0V (vs.SCE)的电位范围内进行循环扫描电活化后 ,维生素B2 在电极表面具有很强的吸附性 ,在底液为 0 .0 5mol/L柠檬酸 + 0 .1mol/LNa2 HPO4 (pH =4 .9)的介质中 ,吸附电位 - 0 .3V ,在 0～ - 0 .7V的范围内 ,以 90mV/s的速度进行循环伏安扫描 .结果 :实验表明VB2 在电极表面具有可逆吸附波的特征 ,VB2 浓度与峰电流在 6.0× 10 - 8～ 7.0× 10 - 6 mol/L之间成线性关系 ,检测限为 1.0× 10 - 8mol/L .结论 :建立了维生素B2 在玻碳电极表面的电化学分析方法 ,将该法运用于实际样品药片中VB2 含量的测定 ,获得满意的结果 .
OBJECTIVE: To study the electrochemical behavior and quantitative analysis of vitamin B2 adsorbed on the surface of glassy carbon electrode.METHODS: The glassy carbon electrode was immersed in 8.0mol / L HNO3 medium at a potential of +0.6 ~ +2.0V (vs.SCE) Within the scope of the cycle of scanning Electro-activation, vitamin B2 in the electrode surface has a strong adsorption in the bottom liquid is 0 0 5mol / L citric acid +0 .1mol / LNa2 HPO4 (pH = 4.9) medium , The potential of adsorption was -0.3V, and cyclic voltammetry was performed at 90mV / s in the range of 0 ~ -0.7V.Results: The results show that VB2 has the characteristics of reversible adsorption wave on the electrode surface, and the peak of VB2 concentration and peak The linear range of the current was 6.0 × 10 -8 ~ 7.0 × 10 -6 mol / L with the detection limit of 1.0 × 10 -8 mol / L.CONCLUSION: The electrochemical method for the determination of vitamin B2 on glassy carbon electrode was established, The method was applied to the determination of VB2 content in the actual sample tablets to obtain satisfactory results.