论文部分内容阅读
以大白菜(Brassica campestris L. ssp. pekinensis)‘丰抗70’为试材,研究了拟康宁木霉SMF2对大白菜软腐病的诱抗效果;并通过研究拟康宁木霉SMF2对接种和未接种主要致病菌——欧氏杆菌胡萝卜软腐亚种(Erwinia carotovora ssp. carotovora)的大白菜幼苗生长、内源激素含量及保护酶活性的影响,探讨了木霉防治大白菜软腐病的机制。结果表明:施用拟康宁木霉SMF2(孢子≥0.1g.kg-1)显著降低大白菜叶柄软腐病病斑长度。无论大白菜幼苗是否接种软腐病菌,施用拟康宁木霉SMF2显著提高大白菜地上部和根系鲜质量、根冠比值,提高叶片玉米素核苷(ZR)、吲哚乙酸(IAA)、脱落酸(ABA)含量,以及多酚氧化酶(PPO)和过氧化物酶(POD)活性。说明拟康宁木霉SMF2可能主要通过促生作用和诱导抗性防治大白菜软腐病。
Chinese cabbage (Brassica campestris L. ssp. Pekinensis) ’Feng 70’ was used to study the inductive effect of Trichoderma koningii SMF2 on the susceptibility to Chinese cabbage soft rot. The effects of Trichoderma koningii SMF2 on inoculation and The main pathogen not inoculated with Erwinia carotovora ssp. Carotovora Chinese cabbage seedling growth, endogenous hormone content and protective enzyme activity to explore Trichoderma control cabbage soft rot disease Mechanisms. The results showed that Trichoderma koningii SMF2 (spores ≥0.1g.kg-1) significantly reduced the leaf spot length of Chinese cabbage soft rot disease. Regardless of cabbage seedlings inoculated with soft rot fungi, Trichoderma koningii SMF2 significantly increased the fresh weight and root-shoot ratio of shoots and roots, increased the contents of ZR, IAA, (ABA) content, as well as polyphenol oxidase (PPO) and peroxidase (POD) activity. These results suggest that Trichoderma koningii SMF2 may be used to control soft rot of Chinese cabbage mainly through promoting growth and inducing resistance.