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目的探究不同寄主植物对桑寄生毒性影响的规律性。方法选择桑树、柳树、夹竹桃、马桑4种寄主植物的桑寄生,制备桑寄生提取物,购进雄性SD大鼠72只,随机分为正常组、4种不同寄主来源的桑寄生高剂量组和低剂量组,每组8只。正常组大鼠灌胃蒸馏水,其他各组灌胃相应的桑寄生提取物。记录比较9组大鼠的整体状况、心功能、血常规、肝肾功能。结果高剂量组和低剂量组大鼠较正常组活跃,高剂量组大鼠活跃程度高于低剂量组(P<0.05);柳树桑寄生组大鼠活跃程度优于其他灌注组(P<0.05)。正常组和高剂量组(夹竹桃)、低剂量组(夹竹桃)心率、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)及左心室重量指数比较,差异有统计学意义(P<0.05);其中,高剂量组(夹竹桃)大鼠心率、LVSP、LVEDP高于低剂量组(夹竹桃)(P<0.05)。正常组和高剂量组(马桑)、低剂量组(马桑)大鼠白细胞计数、淋巴细胞分数、红细胞计数、血红蛋白、血小板计数比较,差异有统计学意义(P<0.05);其中,高剂量组(马桑)大鼠白细胞计数、淋巴细胞分数、红细胞计数、血红蛋白、血小板计数高于低剂量组(马桑)(P<0.05)。正常组和高剂量组(桑树)、低剂量组(桑树)大鼠尿素清除率、血尿酸、尿蛋白比较,差异有统计学意义(P<0.05);其中,高剂量组(桑树)大鼠尿素清除率、血尿酸、尿蛋白高于低剂量组(桑树)(P<0.05)。结论不同寄主植物对桑寄生的毒性影响不同,探索寄主植物对桑寄生毒性影响的规律性,规范桑寄生药材寄主植物来源,有助于提高桑寄生药材质量和安全性。
Objective To investigate the regularity of the effects of different host plants on the parasitism. Methods Mulberry, willow, oleander, Masson host plants parasiticus, the preparation of parasiticus extract, male SD rats purchased 72, were randomly divided into normal group, four different host sources of parasitic high Dose group and low dose group, each group of eight. Normal rats were fed distilled water, the other groups were given the corresponding Sanguinea extract. Record the overall status of nine groups of rats, heart function, blood, liver and kidney function. Results The rats in high-dose group and low-dose group were more active than those in normal group, while those in high-dose group were more active than those in low-dose group (P <0.05). The activity of Willow Sang parasitic group was better than that of other groups ). The heart rate, left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and left ventricular mass index in normal group and high-dose group (oleander), low dose group (P <0.05). Heart rate, LVSP and LVEDP were significantly higher in the high-dose group than those in the low-dose group (P <0.05). The white blood cell count, lymphocyte score, erythrocyte count, hemoglobin and platelet count of normal group and high dose group (MA Sang) and low dose group (MA Sang) rats were significantly different (P <0.05) The white blood cell count, lymphocyte fraction, erythrocyte count, hemoglobin and platelet count in the dose group (MA Sang) rats were higher than those in the low dose group (MA Sang) (P <0.05). There were significant differences in urea clearance, serum uric acid and urinary protein between the normal group and the high-dose group (mulberry) and the low-dose group (mulberry) rats (P <0.05) Urea clearance, serum uric acid, urine protein were higher in the low-dose group (mulberry) (P <0.05). Conclusion Different host plants have different effects on the toxicity of Sanguinea parasiticus, exploring the regularity of the host plants’ toxicity to Sanguineous parasites and regulating the source of the Sangania species host plants, which will help to improve the quality and safety of Sanguineae medicinal materials.