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１８９６和１８９８的Ｇ→Ａ变异是乙型肝炎病毒（ＨＢＶ）ＤＮＡ变异的热点，１８９６变异导致ＨＢｅＡｇ翻译中断。这种变异被认为改变了ＨＢＶ的生物学特性，因而与慢性肝炎和重症肝炎相关。我们以人工变异的方法分别使野毒株土拨鼠肝炎病毒ＷＨＶ－８相当于ＨＢＶ１８９６及１８９６和１８９８位点产生Ｇ→Ａ变异，并构建了首尾相连的双体质粒。分别用这些质粒转染ＨｅｐＧ２细胞，结果其ＤＮＡ复制水平和转录水平未见明显差异。说明这种前Ｃ热点变异不影响ＷＨＶ复制。
The G → A mutation of 1896 and 1898 is a hotspot of hepatitis B virus (HBV) DNA variation, and the 1896 mutation results in the interruption of HBeAg translation. This variation is thought to alter the biological properties of HBV and is therefore associated with chronic hepatitis and severe hepatitis. We mutated wild-type woodchuck hepatitis virus WHV-8 to G18A at positions 1896 and 1898, respectively, and constructed two-piece plasmids that were linked end-to-end. HepG2 cells were transfected with these plasmids respectively. As a result, no significant differences were found in DNA replication level and transcription level. This pre-C hot spot mutation does not affect WHV replication.