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目的研究血红素加氧酶-1(HO-1)抑制剂锌卟啉(ZnPP)对自发性脑出血(ICH)大鼠脑水肿和远期脑萎缩情况的影响。方法应用立体定向仪固定大鼠,将自休新鲜动脉血100μL注入大鼠的右侧基底节区制作ICH实验动物模型,ICH大鼠分为ZnPP治疗组和DMSO溶剂对照组。部分大鼠在术后3 d断头取脑,采用干湿法测定脑组织含水量；部分大鼠在术后28 d灌注取脑作肜态学分析。结果ICH后3 d,ZnPP治疗组同侧基底节脑组织含水量明显低于DMSO对照组(P＜0．05)。ZnPP治疗组两侧基底节面积差值明显小于DMSO对照组(P＜0．05)。结论ICH后腹腔内应用HO-1抑制剂ZnPP缓释泵町以减轻ICH大鼠脑水肿程度和远期的脑萎缩。
Objective To investigate the effects of heme oxygenase-1 (HO-1) inhibitor ZnPP on cerebral edema and long-term brain atrophy in rats with spontaneous cerebral hemorrhage (ICH). Methods Stereotactic instrument was used to immobilize rats. 100 μL of fresh arterial blood was injected into right basal ganglia of rats to make ICH experimental animal model. ICH rats were divided into ZnPP treatment group and DMSO solvent control group. Some rats were decapitated 3 days after the operation, the brain water content was measured by the wet and dry method. Some rats were subjected to morphological analysis by brain perfusion 28 days after the operation. Results At 3 days after ICH, the water content of ipsilateral basal ganglia in ZnPP group was significantly lower than that in DMSO control group (P <0.05). The differences in basal ganglia area on both sides of ZnPP group were significantly less than those in DMSO control group (P <0.05). Conclusions After intraperitoneal ICH, HO-1 inhibitor ZnPP was used to relieve brain edema and long-term brain atrophy in ICH rats.