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本实验应用碱性凝胶电泳技术探讨了活性氧自由基在紫外线照射人体皮肤形成环丁烷嘧啶二聚体中的作用。人皮肤ＤＮＡ加抗氧化剂后，经紫外线照射，Ｔ４Ｎ５切割，电泳，最后进行凝胶成像系统分析。结果可见，抗氧化剂对环丁烷嘧啶二聚体的形成起到一定的抑制作用，尤其是β胡萝卜素和超氧化物歧化酶（ＳＯＤ）比谷胱甘胱（ＧＳＨ）和过氧化氢酶（ＣＡＴ）的作用明显，但均未能完全阻止ＣＰＤ的形成，这提示活性氧自由基在ＣＰＤ的形成中起部分的介导作用。
In this study, the use of alkaline gel electrophoresis technique to explore the role of reactive oxygen species in the formation of cyclobutane pyrimidine dimer UV irradiation of human skin. Human skin DNA plus antioxidants, the UV irradiation, T4N5 cut, electrophoresis, and finally gel imaging system analysis. The results showed that the antioxidants on cyclobutane pyrimidine dimer formation has played a certain role, in particular, β-carotene and superoxide dismutase (SOD) than glutathione (GSH) and catalase (CAT) have obvious effect, but none of them can completely prevent the formation of CPD, which suggests that reactive oxygen species play a partial mediating role in the formation of CPD.